Minimum sample volume: 12µL (3-5 sample concentrations required) The Malvern Zetasizer range provides both exceptionally high performance and entry level systems that incorporate combinations of a particle size analyzer, zeta potential analyzer, molecular weight analyzer, protein mobility and microrheology measurements.Minimum sample volume: 150 µL (20 µL using diffusion barrier method).The migration and invasion of hBMSCs were evaluated with a Transwell assay. the CCK-8 assay, KI67 staining, and a cell cycle distribution assessment. Measurement range: 3.8 nm – 100 µm (diameter) The size of exosomes was measured using a Zetasizer Nano series-Nano-ZS (Malvern Instruments, Worcestershire, UK) according to the manufacturers directions.
#Alvern instruments zetasizer nano series zen3600 software
Measurement range: 0.3 nm – 10.0 µm (diameter) Dynamic light-scattering (DLS) measurements were performed for SEC-EVs and peptide EVs samples using a Malvern Zetasizer Nano series, ZEN3600 and analyzed using Malvern Zetasizer 7.10 software (Malvern Instruments Ltd., Worcestershire, UK).From this the average molecular weight and second virial coefficient A 2can be calculated, which gives a measure of molecule solubility. In this technique, the scattering intensity of a number of concentrations of the sample is measured, and used to construct a Debye plot. The S90 and ZS90 instruments use optics that have a 90° scattering detectorangle for size. Static Light Scattering is used to determine the molecular weight of proteins and polymers. GrovewoodRoad,Malvern, Worcestershire,WR141XZ, UnitedKingdom Tel+441684892456 Fax+441684892789.
An available surface zeta potential accessory uses tracer particles to measure electro-osmosis close to a sample surface to calculate the zeta potential of the surface. An electric field is applied to a solution of molecules or a dispersion of particles, which then move with a velocity related to their zeta potential.